Glycobiology and Glycobiochemistry Services

Laboratory of Glycobiochemistry

Welcome & What can we offer

GlycoLab focuses on glycobiology of ticks and tick-borne pathogens, as well as protein-saccharide interactions important for the process of tick feeding and infection of tick vector and the vertebrate host. Laboratory members participate on projects studying the glycobiology of other organisms as well. Furthermore, bio-activation of surfaces and deposition of bioactive molecules in plasma polymer films is studied. We offer services related to glycobiology and glycobiochemistry for all types of organisms, including MS identification of proteins and glycans.

GlycoLab is situated in the building C of the Faculty of Science, University of South Bohemia in České Budějovice and administrated by the Institute of Chemistry and Biochemistry.

How can you access

General questions

Pavlína Věchtová, vechtp00@prf.jcu.cz

Ján Štěrba, sterbj02@prf.jcu.cz

phone: +420 777 6220

http://www.prf.jcu.cz/en/uchbch/research/laboratory-of-glycobiochemistry.html

Head: Ján Štěrba, sterbj02@prf.jcu.cz

MS operator: Dmitry Loginov

Staff: Pavlína Věchtová, Dmitry Loginov, Pavlína Kočová,

Customer registration

Please, fill in the short registration form

The information will be used (i) for your identification as a customer of our infrastructure (ii) for contacting you in case we will need more information about your samples.

Analytical services

Glycolab provides a wide range of glycobiology analyses. Samples provided by the customer can be analysed or sample manipulation and preparation can be accomplished in the laboratory as well. The exact preparation of the sample and sample requirements for analyses must be consulted prior their submission.

Glycan identification using Schiff staining and lectinoblotting

The presence of glycoproteins can be assessed in any material by Schiff staining and additional analysis using Western blot, fluorescent microscopy etc. More exact determination of the glycan nature can be accomplished by lectinoblotting using a range of lectins. The identified proteins can be further purified or identified by mass spectrometry. Also, glycan structures can be determined using mass spectrometry. Cells containing glycoproteins can be specifically labelled by Schiff staining or lectins and enriched using a cell sorter.

Glycoprotein and glycan purification and identification

Glycan or glycoprotein purification from a complex sample can be performed using chemical immobilization or lectin affinity chromatography. Several approaches are possible depending on the analyses following the purification.

Bioorthogonal labelling and host-vector-pathogen interactions

Bioorthogonal labelling of proteins, glycans, or lipids can be performed in cell cultures or also in vivo (e.g. using artificial feeding system for ticks). This metabolic labelling provides a labelling technique most similar to the natural conditions. The bioorthogonally labelled molecules can be further detected, purified, and identified using Click reaction with a selected reaction partner (wide choice of commercially available molecules including fluorescent dyes).

Glycobiology of infectious pathogens

GlycoLab has access to BSL2 laboratory space and BSL3 laboratory is also available and thus all the above mentioned services can be provided for infectious organisms as well.

 

Laboratory of Glycobiochemistry

University of South Bohemia

Head: Dr. Jan Sterba

http://www.prf.jcu.cz/en/uchbch/research/laboratory-of-glycobiochemistry.html

sterbj02@jcu.cz

Profile:

Our laboratory focuses on glycobiology of ticks and tick-borne pathogens, as well as protein-saccharide interactions important for the process of tick feeding and infection of tick vector and the vertebrate host. Laboratory members participate on projects studying the glycobiology of other organisms as well.

 Selected Publications:

  • Villar M, Ayllon N, Kocan KM, Bonzon-Kulichenko E, Alberdi P, Blouin EF, Weisheit S, Mateos-Hernandez L, Cabezas-Cruz A, Bell-Sakyi L, Vancova M, Bily T, Meyer DF, Sterba J, Contreras M, Rudenko N, Grubhoffer L, Vazquez J, de la Fuente J, 2015: Identification and characterization of Anaplasma phagocytophilum proteins involved in infection of the tick vector, Ixodes scapularis. PLOS ONE 10: e0137237.
  • Vancova M, Sterba J, Dupejova J, Simonova Z, Nebesarova J, Novotny MV, Grubhoffer L (2012: Uptake and incorporation of sialic acid by the tick Ixodes ricinus. JOURNAL OF INSECT PHYSIOLOGY 58:1277-87.
  • Sterba J, Vancova M, Rudenko N, Golovchenko M, Tremblay TL, Kelly JF, MacKenzie CR, Logan SM, Grubhoffer L (2008): Flagellin and outer surface proteins from Borrelia burgdorfeti are not glycosylated. JOURNAL OF BACTERIOLOGY 190:2619-23.

C4Sys Open Access Infrastructure: (complete list)

  • Autoflex speed MALDI-TOF/TOF mass spectrometer (Bruker) (Protein Science Instrument)
  • nanoAcquity UPLC-ESI-QTOF Premier mass spectrometer (Waters) (Protein Science Instrument)
  • Ultimate 3000 RSLC HPLC-LTQ Velos Pro mass spectrometer (Thermo Scientific) (Protein Science Instrument)
  • Trace 1310 GC-TSQ 8000 mass spectrometer (Thermo Scientific) (Analytical chemistry instrument)
  • Trace 1310 GC with ECD and FID detection (Thermo Scientific) (Analytical chemistry instrument)
  • IEF 100 (Hoefer), TransBlot (Bio-Rad) (Protein Science Instrument)
  • Bioanalyzer Lab-on-a-Chip (Agilent) (Protein Science Instrument)
  • J-715 spectropolarimeter (Jasco) (Biophysical Instruments)
  • Alliance 4.7 gel documentation system (Uvitec) and Syngene G:BOX Chemi XX6 (Protein Science Instrument)
  • Ultimate 3000 HPLC (Thermo Scientific), Alliance HPLC (Waters), Prominence LC-20A HPLC (Shimadzu) (Analytical chemistry instrument)
  • FreeZone Plus lyofilizer and Centrivap speed-vac with cold trap (Labconco) (Protein Science Instrument)
  • Stansted Pressure Cell/Homogeniser AO412 (Stansted Fluid Power) (Protein Science Instrument)

Bioinformatics Software

Bioinformatics Databases

Protein Science Instruments

  • Autoflex speed MALDI-TOF/TOF mass spectrometer (Bruker) (Protein Science Instrument)
  • nanoAcquity UPLC-ESI-QTOF Premier mass spectrometer (Waters) (Protein Science Instrument)
  • Ultimate 3000 RSLC HPLC-LTQ Velos Pro mass spectrometer (Thermo Scientific) (Protein Science Instrument)
  • IEF 100 (Hoefer), TransBlot (Bio-Rad) (Protein Science Instrument)
  • Bioanalyzer Lab-on-a-Chip (Agilent) (Protein Science Instrument)
  • Alliance 4.7 gel documentation system (Uvitec) and Syngene G:BOX Chemi XX6 (Protein Science Instrument)
  • FreeZone Plus lyofilizer and Centrivap speed-vac with cold trap (Labconco) (Protein Science Instrument)
  • Stansted Pressure Cell/Homogeniser AO412 (Stansted Fluid Power) (Protein Science Instrument)

Biophysical Instruments

  • J-715 spectropolarimeter (Jasco) (Biophysical Instruments)

Analytical chemistry instrument

  • Trace 1310 GC-TSQ 8000 mass spectrometer (Thermo Scientific) (Analytical chemistry instrument)
  • Trace 1310 GC with ECD and FID detection (Thermo Scientific) (Analytical chemistry instrument)
  • Ultimate 3000 HPLC (Thermo Scientific), Alliance HPLC (Waters), Prominence LC-20A HPLC (Shimadzu) (Analytical chemistry instrument)

Cell Biology Instruments

CATEGORIES:

  • Bioinformatics Software
  • Bioinformatics Databases
  • Protein Science Instruments
  • Biophysical Instruments
  • Molecular Biology Instruments
  • Cell Biology Instruments
  • … add more categories as needed
  • Analytical chemistry instrument
  • Animal facility